RAD Data comm FLM-1 Handbücher

Rad data comm FLM-1 Betriebsanweisung (401 Seiten)

Marke: Rad-data-comm | Kategorie: Software | Größe: 4.64 MB |

Englisch

Inhaltsverzeichnis

Quantity One

1

Table of Contents

3

Contents

5

1. About This Document

9

2. Bio-Rad Listens

10

1. Introduction

11

1.3 About Gel Quality

13

Acquire Image

14

Optimize Image

14

1.5 Computer Requirements

15

Macintosh

16

1.6 Installing on a PC

17

1.7 Installing on a Macintosh

18

1.8 Starting the Program

19

1.8.a Software License

20

(If Necessary)

20

Introduction

21

1.8.c Trial Period

22

1.8.d Software Registration

24

Registering by Internet

25

Registering by Fax or E-mail

26

Entering a Password

26

1.9 Contacting Bio-Rad

27

2. General Operation

29

2.1.b Main Toolbar

30

2.1.c Status Boxes

30

2.1.d Secondary Toolbars

31

2.1.e Quick Guides

32

General Operation

33

2.2 Mouse-assignable Tools

34

2.3 Keyboard Commands

35

Opening TIFF Images

38

Close All

39

Save All

40

Revert to Saved

40

Image Info

40

Change the Image Dimensions

41

Reduce File Size

42

2.5 Preferences

45

2.5.a Misc

46

2.5.b Toolbar

47

2.5.c Application

49

2.5.d Display

50

2.6 User Settings

51

3. Gel Doc

53

3.2 Step I. Position Gel

56

3.3 Step II. Acquire Image

57

Manual Expose

58

Fig. 3-5. Manual Expose

59

3.4 Step III. Select Output

60

3.5 Image Mode

61

3.6 Exposure Status

62

3.7 Display

62

3.8 Options

64

DAC Settings

65

Imaging Area

65

Auto Exposure Threshold

65

Reminder

65

Save Options

66

4. Chemi Doc

67

Chemi Doc

69

4.2 Step I. Position Gel

70

4.3 Step II. Acquire Image

71

4.4 Step III. Select Output

75

Video Print

76

Annotate

76

4.5 Image Mode

77

4.6 Exposure Status

78

4.7 Display

78

4.8 Options

80

5. GS-700 Imaging

83

5.1 GS-700 Acquisition Window

84

GS-700 Imaging Densitometer

85

5.3 Step II. Select Scan Area

88

Selecting an Area

89

5.5 Acquire the Image

91

5.6 Calibration

92

5.6.a Editing the Step Tablet

93

5.7 Other Options

96

6. GS-710 Imaging

99

6.1 GS-710 Acquisition Window

100

GS-710 Imaging Densitometer

101

Quantity One User Guide

102

Choosing Your Own Settings

104

6.3 Step II. Select Scan Area

105

6.5 Calibration

107

6.5.a Editing the Step Tablet

108

Diffuse Versus Specular O.D

110

6.6 Acquire the Image

111

6.7 Other Options

112

Highlight Saturated Pixels

113

Hide Grid

113

7. GS-800 Imaging

115

Densitometer

115

7.1 GS-800 Acquisition Window

116

GS-800 Imaging Densitometer

117

7.3 Step II. Select Scan Area

120

7.5 Calibration

122

7.5.a Editing the Step Tablet

123

7.5.b Calibration Settings

125

7.6 Acquire the Image

126

7.7 Other Options

126

Auto Save After Scan

127

Make Backup Copy

127

8. Fluor-S MultiImager

129

Simulation Mode

130

Fluor-S MultiImager

131

Custom Applications

133

8.3 Step II. Position/Focus

134

Position

135

8.5 Acquire the Image

138

8.6 Options

139

8.6.a Dark Subtraction Type

140

Referenced

141

Reset Reference

141

8.6.b Live Acquire Mode

142

8.6.c Save

143

8.7 Other Features

144

File Size of Images

145

9. Fluor-S MAX MultiImager

147

Fluor-S MAX MultiImager

149

Scan Dimension

152

9.3 Step II. Position/Focus

153

9.5 Acquire the Image

156

Live Acquire

157

9.6 Options

158

Reset Reference Button

159

9.6.b Live Acquire Mode

160

9.6.c Save

161

9.6.d Imaging Area Size

162

9.7 Other Features

163

10.Personal Molecular

165

Imager FX

165

Personal Molecular Imager FX

167

10.2 Step I. Select Scan Area

168

10.4 Acquire the Image

170

10.5 Options

171

11. Molecular Imager FX

173

11.1 FX Acquisition Window

174

Molecular Imager FX

175

Standard Applications

176

Standard FX Applications

176

Sample Intensity

178

11.5 Acquire the Image

183

11.6 Options

184

12. Viewing and Editing

187

Zoom Box

188

Viewing and Editing Images

189

Zoom In/Zoom Out

189

Arrow Keys

189

View Entire Image

190

Centering an Image

190

Imitate Zoom

190

Tiling Windows

190

12.2 Density Tools

191

Density in Box

192

Plot Density Distribution

192

Plot Cross-section

192

Plot Vertical Trace

193

12.4 Multi-Channel Viewer

194

Viewing Options

195

12.5 Image Stack Tool

196

12.6 Colors

198

Changing a Color

199

12.7 Transform

201

12.7.a Transform Subwindows

202

12.7.b Transform Controls

203

Gamma Slider

205

12.7.c Other Features

206

12.8.a Cropping Images

208

Flipping

209

90° Rotations

210

Custom Rotation

210

Auto-scale

214

Dark Contrast Slider

214

Background Slider

215

Background Box

215

Background Stripe

216

Completing the Subtraction

216

12.10 Filtering Images

217

12.10.a Filter Wizard

218

Step 2: Select Filter Size

219

Step 3: Begin Filtering

220

12.11 Invert Data

222

12.12 Text Overlays

223

Editing a Text Overlay

224

Line Tool

224

12.14 Sort and Recalculate

226

13. Lanes

227

13.1.a Creating a Lane Frame

228

Manual Frame Lanes

229

13.1.b Editing the Frame

230

Adjusting the Entire Frame

231

Adding and Adjusting Anchors

231

13.1.c Deﬁning a Single Lane

233

13.1.d Adjusting Single Lanes

233

13.1.f Deleting Lanes

234

13.1.g Lane Width

234

13.1.h Creating a Lane Proﬁle

235

Lanes to Model

237

Trace Display

237

When to Apply

237

Calculation Method

237

13.3 Compare Lanes

239

Magnifying the Graph

240

Show Gaussian Modeling

240

Align Band Types

241

Printing and Exporting

242

13.4 Lane-based Arrays

243

Adjusting the Array Matrix

244

Analyzing Array Data

246

14. Bands

247

Quantiﬁed

248

14.2.a Detection Parameters

251

Shadow Rejection

252

Band Limit

252

Sensitivity

252

Lane Width

253

Minimum Density

254

Noise Filter

254

Shoulder Sensitivity

255

Size Scale

255

Saving Parameters

255

Loading Parameters

256

Deleting Parameters

256

Resetting Parameters

256

In Brackets Mode

257

In Lines Mode

258

14.3.b Adjusting Bands

259

14.4 Plotting Traces of Bands

260

14.5 Band Attributes

261

14.7 Gauss-Modeling Bands

267

Incorrect Modeling

270

One pixel enlarged to

271

14.8.a Contouring Bands

272

14.8.b Drawing Tools

273

Density in Region

274

Drawing Band Boundaries

274

Editing Band Boundaries

276

15. Standards and Band

277

Matching

277

Standards and Band Matching

279

Selecting Predeﬁned Standards

279

Creating New Standards

279

15.1.a Standards Form

281

Entering Standard Values

282

Showing the Modeling Lines

284

Standards Regression Models

286

Display Options

287

Read-Only Standards

288

15.2 Band Matching

289

15.2.b Band Set Form

292

List of Band Types

294

Band Set Toolbar

295

Other Band Set Form Functions

296

15.2.e Graphs of Match Data

297

15.3 Quantity Standards

299

15.3.b The Calibration Curve

304

Checking the Imported Curve

308

16. Volume Tools

309

Volume Contour Tool

310

Volume Tools

311

Volume Freehand Tool

311

Volume Rect Tool

312

Volume Circle Tool

312

Volume Labels

313

Displaying Volumes

314

16.3 Volume Standards

315

Local Background Subtraction

317

Global Background Subtraction

318

16.5 Volume Arrays

319

What Is a Volume Array?

320

Creating a Volume Array

320

Moving an Array

322

Resizing an Array

322

Resizing the Array Cells

323

Copying an Array

323

Ungrouping an Array

324

17. Colony Counting

325

Colony Counting

327

17.2 Counting the Colonies

328

17.3 Displaying the Results

329

Colonies

330

White and Blue Colonies

331

17.8 Saving to a Spreadsheet

334

Creating/Opening a Batch File

335

Naming/Saving a Count

336

Loading Another Image

336

18. Differential Display and

337

Normalization

338

Displaying the Results

339

Testing for Ambiguity

341

19. Reports

343

Printing Your Report

345

Exporting Your Report Data

346

19.2 Lane and Match Reports

347

19.3 1-D Analysis Report

349

Comparison Options

350

Comparison Method

351

19.4.a Compare Lane Images

352

19.4.b Phylogenetic Tree

354

Neighbor Joining

356

Other Methods

356

Neighbor Joining Only

359

All Other Methods

359

19.4.c Similarity Matrix

360

19.5 Volume Analysis Report

361

19.5.a Volume Report Options

362

Background Subtraction Method

363

19.6 Volume Regression Curve

364

19.7 VNTR Report

366

20. Printing and Exporting

369

20.1.b Print Actual Size

371

20.1.d Video Printing

373

Analysis Export Mode

374

Publishing Export Mode

374

Exporting the Image

375

Appendix A

377

Appendix B

379

B.2 Gel Layout

381

Appendix B. Other Features

383

Zoom tools

401

RAD Data comm Geräte